Journal: Scientific Reports

Article Title: Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis

doi: 10.1038/s41598-021-88133-7

Figure Lengend Snippet: Tiliroside restrained the 3D formation ability and CD133 expression of Hep3B and SNU-449 cells. The representative 3D spheroid models of Hep3B ( A ) and SNU-449 ( B ) cells treated by 40 μM Tiliroside and NC. The relative cross-section area formation efficiency showed significant reduction of area of Hep3B ( C ) and SNU-449 ( D ) in Tiliroside groups. Relative inhibition rates of Hep3B and SNU-449 in response to Tiliroside were calculated by comparing the fluorescence value of Tiliroside to NC, at 24, 48, 72, 96 and 120 h, respectively ( E ). Tiliroside (40 μM) treatment significantly reduced the CD133 relative expression levels in both 3D cultured Hep3B and SNU-449 cells (P < 0.001) ( F ). NC negative control.

Article Snippet: The primers for CD133 were: forward, 5′-GATTCATACTGGTGGCTGGGTGG-3′ and reverse, 5′-GCAGGTGAAGAGTGCCGTAAGT-3′ , and carbonic anhydrase XII qPCR primer pairs(human) were obtain for Sino Biological (CN) (Cat: HP100073).

Techniques: Expressing, Inhibition, Fluorescence, Cell Culture, Negative Control

Journal: bioRxiv

Article Title: Synthesis, anticancer properties, and biological profiling of synthetic glycan analogs of proscillaridin A

doi: 10.1101/2025.03.31.646140

Figure Lengend Snippet: Flow cytometry profiling of key markers and cell cycle analysis in human and murine colon cancer cells. A) HCT-116 cells treated with 1 μM of compound 5 exhibit significantly enhanced surface expression of cleaved caspase-3, the hallmark of pre-apoptotic cell death. B) Effect of compounds 1 through 5 on surface CD133 (prominin 1) expression. C) Effect of compounds 1 through 5 on surface CD117 (c-Kit) expression. D) Cell cycle analysis by flow cytometry on HCT-116, HT-29, and CT26 cells treated with 100 nM of compounds 1 through 5

Article Snippet: Cleaved caspase-3 anti-mouse monoclonal antibody (Cat. #Ab156544) and CD117/c-Kit anti-mouse monoclonal antibody (Cat. #Ab096639) were obtained from Aladdin Scientific Corp. Anti-CD133 monoclonal antibody HB#7 was obtained from the DSHB and deposited there by Swaminathan, S.K./Panyam, J./Ohlfest, J.R. Ribonuclease A was obtained from Research Products International (97% purity).

Techniques: Flow Cytometry, Cell Cycle Assay, Expressing